cloning and expression of ebi3 and p28 subunits of human interleukin 27 in e. coli

background: interleukin-27 is a heterodimeric cytokine belonging to il-12 and il-23 families, secreted  by antigen presenting cells( apcs ) .the il-27 is composed of 2 subunits: epstein-barr virus induced gene 3 (ebi3) and p28. il-27 is an anti-inflammatory cytokine which has an inhibitory effect on th17 population and suppress the il-17 expression. it is suggested that il-27 could be a potent drug candidate for treating auto immune diseases. methods: the il-27 subunit genes were constructed into plasmid vectors, they sub-cloned into petdeut-1an expression vector in restriction sites of bamhi, saci and noti. subsequently the induction was done by iptg and the recombinant proteins were confirmed by sds-page and western blot analysis using anti his-tag antibody. results: the 28kda and 25kda protein bands were observed on sds-page and finally confirmed by western blot technique.